The presence of various heavy metals, including hazardous metals, in Hochiminh City's Pleistocene aquifer has recently been reported. The ANOVA approach (analysis of variance) was used in conjunction with monitoring data and field surveys to investigate the effects of anthropogenic activities on heavy metal levels. The findings indicate that anthropogenic activities have an impact on the distribution of heavy metals such as Ni, Cu, Cd, and Pb. There was a statistically significant difference of these metal levels between the monitoring wells. Although the levels of these metals remain low, they must be monitored on a regular basis, notably for Cd and Pb. The other heavy metals, Al, Cr, Fe, Mn, and Zn, showed natural backgrounds and insignificant impacts from anthropogenic activities. It proved that one-way ANOVA was effective in carrying out hydrogeochemical data analysis. This study results provided scientific baseline for policy makers to effective manage groundwater in Hochiminh City.