Hepatocellular carcinoma (HCC) is one of the most common cancer worldwide. Many present methods were applied with low efficiency. Therefore, new methods are being studied for treating this cancer. It is necessary to store HCC tumors to isolate cancer cells. So, this study aims to establish a protocol to cryopreserve the HCC tumors. HCC tumors were transferred to laboratory at cool condition. The tissues were dissected into pieces 2x2x2 mm3 and stored in cryopreservation medium with 10 percent DMSO. Two cooling down processes were investigated in this research, included: three-step protocol and ultra-rapid cryopreservation. After 1.5, 3 and 5 months, tumors were thawed by quick-thaw method. Cell viability in the tumor was estimated by trypan blue based on counting. Then viable tumors were cultured to determine the cellular expansion. The result showed that the viable cell rate decreased gradually after 1.5, 3 and 5 months of cryopreservation with 3783 percent, 0.31 percent and 0 percent by the three-step protocol and 8.29 percent, 0.19 percent and 0 percent by the ultrarapid cryopreservation, respectively. Both methods had low efficiency when tumors were cryopreserved more than 3 months. However, these results also showed that it is possible to cryopreserve HCC tumors for further research and application.
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