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Công nghệ sinh học công nghiệp

Nguyễn Thanh Sang; Nguyễn Bá Nam; Hoàng Thanh Tùng; Nguyễn Phúc Huy; Nguyễn Thị Kim Loan; Nguyễn Ngọc Thảo; Vũ Đức Trung; Nguyễn Văn An; Trần Thị Minh Loan; Nguyễn Văn Kết; Dương Tấn Nhựt; Hoàng Thanh Tùng(1); 

Sinh trưởng, phát triển và hàm lượng Chlorophyll trong chồi cây cúc (Chrysanthemum morifolium Ramat. CV. "Jimba" nuôi cấy In vitro dưới ánh sáng LED

Growth, development and chlorophyll contents of Chrysanthemum (C. Morifolium Ramat. cv. "Jimba") shoots cultured in in vitro under light-emitting diode

Công nghệ sinh học

2014

2

339-347

1811-4989

Chlorophyll; Sinh trưởng; Phát triển; Hàm lượng; Cây cúc; Chrysanthemum morifolium; Nuôi cấy In vitro; ánh sáng LED; Điều kiện chiếu sáng

In this study, the effects of different light conditions on the shoot multiplication; the growth and development as well as the contents of chlorophyll a and b of Chrysanthemum cultured in vitro were investigated. Chrysanthemum shoots were cultured under different light conditions, specifically red LED, blue LED, yellow LED, green LED, white LED, red and blue LED in combination (R:B = 10:90, 20:80, 30:70, 40:60, 50:50, 60:40, 70:30, 80:20, 90: 10, respectively). After 4 weeks of culture, the results indicated the best light condition for shoot multiplication was 50 percent red LED plus 50 percent blue LED. The combination of 70 percent red LED and 30 percent blue LED gave the best results in term of fresh weight, dry weight, length and width of leaf (1.25 g; 0.07 g; 1.72 cm; 1.60 cm; respectively). In addition, the contents of chlorophyll a, b depended on different light conditions. The highest contents of chlorophyll a (29.20 ug/cm2) and chlorophyll b (14.57 ug/cm2) were obtained under 70 percent red LED and 30 percent blue LED. Thus, the best shoot multiplication was obtained under 50 percent red LED plus 50 percent blue LED; however, the combination of 70 percent red LED and 30 percent blue LED were optimal for the growth and development as well as the contents of chlorophyll a and b of Chrysanthemum.

TTKHCNQG, CVv 262

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