



Cây công nghiệp và cây thuốc
Vũ Xuân Tạo; Trần Bảo Trâm; Nguyễn Thị Mến; Trương Thị Chiên; Trần Bình Minh; Thái Hạnh Dung; Trần Văn Tuấn; Nguyễn Quang Huy; Trần Văn Tuấn(1); Nguyen Quang Huy(2);
Nghiên cứu xác định gen giới tính MAT, năng suất và hàm lượng cordycepin của một số chủng nấm dược liệu Cordyceps militaris đang được nuôi trồng tại Việt Nam
Study on determining the MAT sex gene, yield and cordycepin content of some strains of the medicinal mushroom Cordyceps militaris being cultivated in Vietnam
Khoa học & công nghệ Việt Nam
2022
7B
60 - 64
1859-4794
Chủng nấm; Dược liệu; Nuôi trồng; Gen giới tính MAT; Năng suất
Cordycepin; Cordyceps militaris; MAT1-1-1; MAT1-2-1; Yield
Cordyceps militaris is a precious medicinal mushroom and is being cultivated on a large scale in Vietnam. However, the cultivation of this fungus is facing problems with seed degeneration, which reduces the yield and content of cordycepin in the fruiting body. The MAT sex gene system plays an important role in the formation of fruit bodies in C. militaris. The data on the MAT sex gene and the yield and quality of C. militaris strains being cultivated in Vietnam is an important basis for research and solutions to overcome the problem of seed degeneration. . This study isolated and identified 8 strains of C. militaris from fungal fruiting samples grown in Vietnam based on morphological characteristics and ITS region sequences of rDNA. Eight fungal strains have been identified with sex genes MAT1-1-1 and MAT1-2-1, specifically: 5 fungal strains (QN6, HL11, HL12, TN01 and TN02) carry both MAT1-1-1 sex genes. and MAT1-2-1, three fungal strains (SH03, HL13 and HN01) carry only the sex gene MAT1-1-1. All 8 fungal strains have the ability to form fruit bodies, in which strain C. militaris SH03 carrying single sex gene (MAT1-1-1) gives the highest yield and quality (average fresh fruit body yield is 31). g/box, biological yield of 13.77% and cordycepin content of 12.5 mg/g of dried fruit body), has potential for application in production.
TTKHCNQG, CVv 8