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Bảo vệ thực vật

Nguyễn Đức Thành; Trịnh Xuân Hoạt; Mai Văn Quân; Hà Viết Cường; Hà Viết Cường(1); 

Xác định phytoplasma nhóm 16SRII-A gây hại trên cây sắn tại Đông Nam Bộ bằng kỹ thuật PCR, RFLP

Molecular identification of a 16Srll-A phytoplasma associated with witches' broom disease of cassava in South Eastern Region

Bảo vệ thực vật

2015

2

42-49

2304-5710

Cây sắn; Bệnh hại; Phytoplasma; 16SRII-A; Kỹ thuật PCR; Kỹ thuật RFLP; Nghiên cứu

In recent years, witches' broom disease of cassava is one of the most serious disease in the South Eastern region. The infected plants showing typical witches' broom symptoms were observed on cassava: small leaves, yellowing and formation of excessive buds. Total DNAs were extracted from the symptomatic and the asymptomatic cassava plants. Using universal primer P1/P7 followed by R16mF2/R16mR1 by nested polymerase chain reaction assay to detect the presence of phytoplasma in symptomatic cassava plants. No amplification products were obtained from templates of asymptomatic. PCR products were sequenced directly and the result of BLAST search in the GenBank confirmed that the nucleotide sequences had 99 percent identity with several members of the peanut witches' broom group. Virtual restriction fragment length polymorphism pattern and phylogenetic analysis showed that phytoplasma associated with diseased cassava plants belongs to the subgroup A of the peanut witches' broom group. This is the first report of a 16Srll-A phytoplasma infecting cassava in Vietnam.

TTKHCNQG, CVv 64

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