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 Lượt truy cập :  14,859,285

62.33

Công nghệ sinh học công nghiệp

Trịnh Thị Lan Anh; Nguyễn Quốc Hiệu; Nguyễn Thị Kim Loan; Nguyễn Thanh Sang; Hoàng Thanh Tùng; Võ Thị Bạch Mai; Dương Tấn Nhựt; Hoàng Thanh Tùng(1); 

Cảm ứng tạo mô sẹo có khả năng sinh phôi làm nguồn mẫu cho phát sinh phôi vô tính và nuôi cấy huyền phù tế bào lan hồ điệp (Phalaenopsis amabilis)

Embryogenic callus induction for somatic embryogenesis and cell suspension cultures of Phalaenopsis amabilis

Công nghệ Sinh học

2014

3

531-541

1811-4989

Huyền phù tế bào; Mô sẹo; Sinh phôi; Phát sinh phôi vô tính; Lan hồ điệp; Nghiên cứu

In the present study, the influence of factors on embryogenic callus induction, somatic embryogenenis and cell suspension culture of Phalaenopsis were investigated. Flower stalk was exploited for setting asceptic culture. Protocorm like bodies obtained from in vitro leaves were used as plant material for embryogenic callus induction. The results indicated that the best callus formation was recorded when PLB longitudinal Thin Cell Layer (lTCL) explants were cultured on medium containing 10 g/I mannitol, and PLB ITCL explants cultured on medium supplemented with 3 mg/I adenine gave the highest callus induction rate. Callus clumps then were sub-cultured in order to examine somatic embryogenesis and cell suspension culture. Medium formulation and sucrose concentration affected the somatic embryogenesis, and Murashige and Skoog (MS) medium with 40 g/l sucrose showed to be the most suitable medium for this process. Among carbohydrate sources (including sucrose, glucose, lactose, maltose, mannitol and starch) were added into the media at the concentration of 40 g/I, glucose was found to be the best for somatic embryogenesis. In addition, liquid shaking culture was also effective for cell suspension culture of Phalaenopsis. The present results are significant in the respect of application of somatic embryogenesis and cell suspension culture in high potential micropropagation of Phalaenopsis.

TTKHCNQG, CVv 262

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