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  • Công bố khoa học và công nghệ Việt Nam

76.03

Công nghệ sinh học trong y học

Chử Văn Mến, Nguyễn Duy Bắc(1), Đặng Grường Giang, Nguyễn Văn Long

Nghiên cứu định lượng lycopen trong cà chua bằng phương pháp sắc ký lỏng hiệu năng cao

Quantitation of lycopene in tomatoes by using HPLC-UV/VIS method

Y học Việt Nam

2014

2

70-74

1859-1868

In this study, the authors developed a new HPLC-UV/VIS method for the quantitation of Lycopene in tomatoes. The separation was carried out on a reversed phase C30column (10 mm x 250 mm, 5 um) with the mobile phase of methyl -tert butyl ether: methanol (75:25, v/v), flow rate of 1.5 mL/min, wavelength of 472 nm. The linear range of the method was 0.02-66.67 ug/mL. LOD and LOQ of the methods were 8,0 and 16,0 ng/mL, respectively. The precision and accuracy of the method were 2.35 percent - 4.87 percent and 97.6 percent - 103.4 percent, respectively. The method was the applied to the quantitation of 20 tomatoes samples purchased from markets, the contents of Lycopene in Tomatoes varied from 3 to 12 mg/100 g based on fresh weight.

TTKHCNQG, CVv 46