BỘ KHOA HỌC VÀ CÔNG NGHỆ
HỆ THỐNG THÔNG TIN KHOA HỌC VÀ CÔNG NGHỆ

Lọc theo danh mục
  • Năm xuất bản
    Xem thêm
  • Lĩnh vực
liên kết website
Lượt truy cập
 Lượt truy cập :  14,924,786

62.37

Công nghệ sinh học công nghiệp

Huỳnh Thị Thu Huệ; Nguyễn Văn Trường; Bùi Mạnh Minh; Đoàn Thị Bích Thảo; Nguyễn Xuân Thắng; Nông Văn Hải; Bùi Mạnh Cường; Huỳnh Thị Thu Huệ(1); 

Thiết kế vector biểu hiện mang gen modiCspB và chuyển gen này vào cây ngô

Construction T1-plasmid harbouring modiCSPB gene and transforming the gene into maize plant

Công nghệ Sinh học

2014

1

125-132

1811-4989

CspB; Modi CspB; Thiết kế vector; Chuyển gen ngô; pCAMBIA1300

Bacillus subtillis derivedgeneCspB, coding for RNA chaperone functional protein enables mRNA stable as single-stranded in adverse conditions. Therefore, the expression CspB protein in transgenic plant could increaseresistant ability of plants to environmental stresses such as cold, heat and drought In order to optimize the expression of the bacterial gene in plants, an artificial- synthesis CspB(modiCspB) gene with 46 nucleotide modifications from AfT to G/C was obtained to achieve a more compatible expression g~ne in plant. ModiCspB gene was inserted in pCAMBIA1300 under the control of 35S promoter and transformed in to immature embryos of three maize inbred lines of Vietnam V152N, C436 and C7N by Agrobacterium-mediated method. The results of transformation obtained 68 regenerated plants survived in the green house, 62.02 percent of total regenerated plants. In which, the line V152N produced the highest (70.37 percent) of transgenic plantsand the lowest was C463 47.83 percent). The transformation efficiency was 0.56 percent. after fertilityassessment and PCR analysis.. As consequence, the research has provided the initial transgenic maize plants by improvementof the stress tolerance.

TTKHCNQG, CVv 262

Xem toàn văn