The experiment was aimed at investigating the growth and anatomical characteristics of Brachiaria mutica (B. mutica). The experiment was designed in completely randomized design with 7 treatments (T): T1 (10 days), T2 (15 days), T3 (20 days), T4 (30 days), T5 (40 days), T6 (50 days) and T7 (60 days). The growth and development of B. mutica at three different stages: (1) the budding stage from T1 to T3, in this period the plant concentrates on shoot regeneration; (2) the period of elongation of internodes and increase of leaf area from T4 to T6, which is the stage of strong growth, including height, stem diameter, length and width of leaves; (3) slow growth stage from T7 (60 days) onwards, at this stage leaf area stops growing. The results of microsurgery showed that in T7, the number of cells walls impregnated with lignin, the primary substance comprise of wood was achieved the highest. The structures of tissues with insoluble fiber such as sclerenchyma and collenchyma of stem in T2 and T4 were similar and lower than in T7. The anatomical images of leaves in T7 also showed a higher number of schlerenchyma as compared with T2 and T4, resulting in a decrease of tissue area capable of anabolic/photosynthetic processes. The optimal combination of yield and quality of B. mutica needs to be considered during the period from 30-60 days after cutting.
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