Study on spiders in urban area of Hanoi were undertaken between September and November 2010. The locations have been chosen include Thongnhat park. Nghiado park, Pasteur park, Hangdau park, and a line of trees along Hoangquocviet road. Thongnhat park and Nghiado park are the locations wpich high diversity of habitat. The other locations are less diverse because of human activities. The collected 447 individuals belong to 10 families and 57 species, among them 46 species (240 adults) from Thongnhat park. 34 species (189 adults) from Nghiado park, 1 species (5 adults) from Hangdau park. 1 species (3 adults) from Pasteur park, and 3 species (10 adults) from a line of trees along Hoangquocviet road. The results showed that the assemblages of spiders were significantly different between locations because of the habitat diversity.