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Lượt truy cập
 Lượt truy cập :  14,852,391

76.03

Công nghệ sinh học trong y học

Quách Ngọc Tùng; Khiếu Thị Nhàn; Chu Kỳ Sơn; Vũ Thị Hạnh Nguyên; Vũ Thu Trang; Phí Quyết Tiến; Vu Thu Trang(1)Quách Ngọc Tùng(2)

Đánh giá và sàng lọc xạ khuẩn nội cộng sinh trên cây quế có khả năng kháng vi sinh vật gây bệnh

Assessment and screening of antimicrobial activities of endophytic actinomycetes associated with Cinnamomum loureiri

Công nghệ sinh học

2014

2

365-371

1811-4989

Kháng sinh; Xạ khuẩn nội cộng sinh; Cây quế; Polyketide; Streptomyces

Endophytic actinomycetes associated with medicinal plants are known as producers of bioactive substances with high commercial value such as antimicrobial, anticancer capabilities. In this study, 78 endophytic actinomycetes were isolated from Cinnamomum loureiri plants collected in Hoa Binh province. Experimental results showed that 16.7 percent of total isolates inhibited the growth of Proteus vulgaris CNLM; the same ratio of 14.1 percent inhibited Escherichia coli ATCC 11105; Sarcina lutea CNLM, Staphylococcus epidemidis ATCC 12228, Candida albicans ATCC 10231; 12.8 percent inhibited Bacillus cereus ATCC 11778 and 1 percent displayed antagonistic activity against Salmonella enterica subsp. enterica serovar Typhimurium ATCC 14028. Among those tested, the isolates HBQI9, HBQ33 showed the highest activity against Staphylococcus epidemidis ATCC 12228, Candida albicans ATCC 10231, respectively. Amplification of genes involved in the biosynthesis of antibiotics including polyketide synthases type I (PKS-I), polyketide synthases type I (PKS-II) va nonribosomal peptide synthetase (NRPS) found that both isolates HBQI9, HBQ33 possessed genes PKS-I, PKS-II and did not have NRPS gene. The description of the morphological, biochemical and physiological properties showed that both isolates HBQ19 and HBQ33 have the spiral chains of spores with the smooth surfaces, no soluble pigments, well-growth at 28-37°C; temperature, pH ranges for growth at 28-37°C, pH 5-11. Besides the ability to utilize different carbon, nitrogen sources, both isolates can synthesize hydrolytic enzymes such as amylase, chitosanase and cellulase. Based on the criteria of International Streptomyces Project (ISP), Bergey's Mannual of Determinative Bacteriology and sequence analysis of gene 16S rDNA, the strains HBQ19 and HBQ33 were classified as Streptomyces ahgustmyceticus HBQ19, Streptomyces graminisoli HBQ33, respectively.

TTKHCNQG, CVv 262