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  • Công bố khoa học và công nghệ Việt Nam

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Công nghệ sinh học công nghiệp

Trần Xuân An, Đặng Xuân Nghiêm, Tăng Thị Hạnh, Phạm Văn Cường, Đỗ Thị Phúc(1)

Nghiên cứu trình tự gen mã hóa cho Lea Protein ở một số giống lúa địa phương chịu hạn

Study on sequences of gene encoding LEA protein in some salt resistant rice varieties

Khoa học và Phát triển

2014

4

516-521

1859-0004

LEA protein comprises a large group of proteins which highly accumulates in plant seeds at late stage of maturation. LEA protein is proven to be involved in abiotic stress responses in plants, such as under dehydration and salinity. Genes encoding LEA proteins are divided into 6 different groups. Lea21 gene belongs to group 5. Up to now, there is no report about sequence polymorphsim of Lea21 gene in Vietnamese rice varieties. Therefore, in order to investigate the genetic diversity in rice, Lea21 gene from some salt resistant rice varieties was amplified and sequenced. The Lea21 gene, which consists of 2 exon and 1 intron, was sucessfully amplified using PCR technique and was sequenced.. It was shown that no difference in nucleotide sequences among investigated rice varities was observed, indicating that the sequence of Lea21 gene is highly conserved.

TTKHCNQG, CVv 261