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Nguyễn Thị Phương Thảo; Lê Thành Long; Văn Thị Hạnh; Nguyễn Thị Hồng Vân; Nguyễn Khắc Duy; Nguyễn Thị Hồng Vân(1)

Đánh giá tính đa dạng di truyền của Baculovirus gây nhiễm sâu hại trên rau ở một số khu vực miền Nam Việt Nam

Genetic relationship of baculovirus infected common pests on vegetables in the Southern of Vietnam accessed by lef-8 gene

Sinh học

2013

4

424-428

0866-7160

Sâu hại; Đa dạng di truyền; Baculovirus; Nghiên cứu; Miền Nam Việt Nam

Polymerase chain reaction was applied to amplify baculovirus-specific lef-8 gene. Lef-8 gene sequences were used to access genetic distances and phylogenetic relationship of baculovirus der'ived from Southern Vietnam and other baculovirus from Genbank. The results showed that almost baculovirus from Southern Vietnam expressed 450bp of lef-8 gene. The genetic distance between baculovirus from Southern Vietnam and Alpha-2 baculovirus was lowest (0.346 + or - 0.069), suggesting that they had close genetic relationship. Neighbor-joining method was applied for phylogenetic construction. Bootstrap analyses (using 1000 replications) were used to access the confidence in branching order. Phylogenetic tree demonstrated that baculovirus from Southern Vietnam and Alpha-2 baculovirus, especially spodoptera_litura_NPV, located in the same clade with 86 percent bootstrap value, suggesting that spodoptera_litura_NPV has wide distribution in Southern Vietnam.

TTKHCNQG, CVv 27