Spirulina (Arthrospira platensis) is a species of Cyanobacteria and is widely cultivated for functional foods, cosmetics and aquaculture feed due to its rich protein, pigments, vitamins, unsaturated fatty acids and trace elements. In Vietnam, Spirulina was first studied in the late 1970s, mainly focused on investigating environmental and nutritive factors as well as culture techniques. However, very few studies on isolation, identification and c-haracterization of native strain have been done. With the aim of exploiting the indigenous genetic resources, a strain of Cyanobacteria distributed in Van Quan lake (Hanoi) was isolated, identified and cultured in the medium of Hoagland, BBM, BG-11, ½ Chu-10 and Zarrouk. By morphological analysis using optical and electron scanning microscopy combined with 16S rRNA sequence analysis, the isolated strain was identified as Arthrospira platensis. As the results attained, except for Zarrouk media, this species can grow well in in BG-11 and BBM media. The study provided an initial scientific basis for exploiting indigenous genetic resources for functional foods, cosmetics and aquaculture feeds.