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Lượt truy cập
 Lượt truy cập :  14,989,897

62.33

Công nghệ sinh học công nghiệp

Đỗ Xuân Đồng; Đỗ Hải Lan; Phạm Bích Ngọc; Lê Văn Sơn; Lê Trần Bình; Chu Hoàng Hà; Phạm Bích Ngọc(1);  Phạm Bích Ngọc(2); 

Nghiên cứu hệ thống tái sinh cây sắn (Manihot Esculenta Crantz) thông qua phôi Soma từ đỉnh chồi

An efficient protocol for plant regeneration of cassava (Manihol esculenta Crantz) via somatic embryogenesis induction

Công nghệ Sinh học

2012

3

527-533

1811-4989

Cây sắn; Nuôi cấy mô; Đỉnh chồi; Phôi soma; Tái sinh; Manihot Esculenta Crantz

A method for the induction of somatic embryogenesis in two cassava genotypes from Vietnam is described. Shoot apexes isolated both from in vitro plants and from in vivo stem cuttings were used as samples. Somatic embryogenesis was achieved in high frequencies by the addition to the induction medium ofthe auxin picloram over a wide range of concentrations. The results show that the highest rate of somatic embryos formation for both cultivars was obtained by using 12 mg/l picloram supplemented to MS media. Although the number of embryos per explant was similar between two cultivars for 4 weeks, the KM94 cultivar gave a higher rate of somatic production (82 + or - 1.7 percent) than the KM 140 cultivar (59.6 + or - 2.9 percent). Somatic embryos were subsequently transferred onto media (MS supplemented with 0.3 mg/l BAP) for the highest frequency of plantlet regeneration (KM 140 - 81 + or - 3.2 percent and KM94 - 79 + or - 2.3 percent). Shoots with the length of about 1 - 1.5 cm were transferred onto free - hormone MS media for 100 percent of rooting for 2 weeks. Complete plantlets were cultivated on a mixture of rice husk and sand-soil under ratio 4:6 in greenhouse. This protocol required 16 to 18 weeks and is entirely appropriate for mass production of various cassava genotypes and further genetic transformation experiments.

TTKHCNQG, CVv262

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