Diperocapus grandiflorus Blco (Dau dot tim) is a species of large timber, evergreen high 30-35m, diameter 50-100cm is being over-exploited, only a single population at Dai Thanh humlet, Dai Loc district, Quang Nam province. Thirty three ISSR primers and four SSR primers were used to analysed genetic diversity of 25 individuals of Dau dot tim collected in Quang Nam. A total 37 polymorphic markers (33 ISSRs and 4 SSRs) were used, there were 31/37 primers successfully cloned. There were 28/31 primers revealing polymorphic with PIC value (Polymorphic Information Content) ranged from 0 (ISSR_ 49, SSR_Shc09 and SSR_Shc11) to 0.398 (ISSR_7). Total of 194 fragments DNA segments with size ranging from 110-3000bp, including 148 polymorphic segments (76.3 percent). Degree of genetic similarity between 25 sample D. grandiflorus ranged from 0.595 (QN6 and QN24) to 0.883 (QN27 and QN28). Tree diagram of the genetic relationship of 25 samples of D. grandiflorus were classified into 2 main groups and showed the genetic variation ranging from 11.5 percent (1-0.885) and 34 percent (1-0.66). The first group included only one QN6 genetic coefficient of variation for 24 different samples of about 34 percent (1-0.66). The second group Included 24 remaining samples, genetic coefficient of variation ranging from 11.5 percent to 31.5 percent (1-0.695). The second group also divided into 2 sub-groups, 11.1 and 11.2. In sub 11.1 including 2 samples (QN29 and QN30), genetic coefficient of variation is 21.5 percent (1-0.785). In sub 11.2 divided into two small sub-branches only small 1:1 sample (QN3), small tributary 2 the remaining 22 samples with a coefficient of genetic vanation from 11.5 percent (1-0.885) to 27 percent (1-0.73).
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