Distribution and antibiotic resistance of bacteria in patients with ENT infection. Methods: A cross sectional study on 72 patients with ENT infection had positive bacterial culture results at the Otorhinolaryngology Department of Cho Ray Hospital. Results: Our study isolated 78 bacteria in 72 patients admitted to our department. The most common bacteria were Staphylococcus aureus (26,9%), Pseudomonas aeruginosa (24,4%), Klebsiella pneumoniae (16,7%). Regarding their antibiogram, we found that Staphylococcus aureus was most sensitive to vancomycin (100,0%), teicoplanin (100,0%), linezolid (94,1%). On the other hand, Pseudomonas aeruginosa was most sensitive to tobramycin (100,0%), imipenem (94,4%), meropenem (94,7%), amikacin (94,4%). Klebsiella pneumoniae was most sensitive to ertapenem (76,9%), meropenem (76,9%), imipenem (75,0%). Conclusion: The results show the pattern bacterial strains and their antibotics resistance in ENT infection. We need to consider the indication of antibiotics according to the antibiogram and limit unnecessary use of antibiotic.