Antibiotics are commonly used against secondary bacterial infections in dogs suffered with parvo disease. The aim of this study was to determine the antibiotic resistance profile of E. coli isolated from dogs infected with parvovirus. The studied result showed that among 25 rectal swab samples from dogs diagnosed with parvo, there were 16 (64%) samples found to be positive with E. coli. The E. coli isolates showed high resistance rate with ampicillin (68.75%), followed by cephalexin (50%), while the lowest resistance rate was observed with amikacin (6.25%). Notably, there were 75% (12/16) of isolates resistant to at least one antibiotic, with 7 resistance patterns. Especially, 25% (4/16) of E. coli isolates were determined as multidrug resistant strains. The phenotypic and genotypic characterization of ESBL-producing E. coli revealed that 4 strains were capable of producing ESBL and 3 genotypic patterns were recorded, including: 1 (25%) isolate carried the blaTEM gene, 1 (25%) isolate haboured the blaCTX-M-1 gene, and 2 (50%) isolates simultaneously possessed both blaTEMand blaCTX-M-1 genes.