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62.41

Công nghệ sinh học công nghiệp

Nguyễn Thị Cúc; Đỗ Thị Thảo; Nguyễn Thị Nga; Đỗ Thị Phương; Nguyễn Thị Trang; Đỗ Thị Thảo(1)

Đáp ứng miễn dịch kháng u của kháng thể nguyên khối u Globo H-KLH trên chuột

Immunological response against tumors by using Globo H-KLH antigen vaccinated on mice

Công nghệ Sinh học

2014

4

623-629

1811-4989

Chuột BALB/c; Đáp ứng miễn dịch; Kháng nguyên Globo H-KLH; Tế bào LLC; Vắc xin

Globo H molecules, which was first isolated from breast cancer MCF-7 cells, are glycan with chemical structure as hexasaccharide. Globo H apprears as an important cancer antigen. Based on its ability to cause immune response, Globo H becomes a promissing target for anti-cancer vaccine researches and development. In order to identify the potential immunological response against tumors of Globo H, in the study, those molecules were firstly conjugated with Keyhole Limpet Hemocyanin (KLH). As the next step, the conjugated Globo H-KLH complex were then vaccinated to albino BALB/c mice at different concentrations which were I, 2 and 4 !tg/mouse/times by exactly following the immunized protocol of Liddell

TTKHCNQG, CVv 262