C7 is one of the important components of the membrane attack complex (MAC) of the complement system, which can cause lysis ofthe target cells. Herein study described cDNA sequence variation of the porcine C7 gene (PC7) obtained in different breeds (Hampshire, German Landrace, Duroc, Pietrain, Berlin Miniature), assigned the pC7 to chromosome as well as evaluated the effect of its genetic variation on the haemolytic complement activity (CH50) and C3c serum concentration (C3c) in a F2 DUMI resource population. In this study, six SNPs, in which three of them had amino acid exchange 724A-G (52Ile-Val, BsrDI), 320C-T (67Thr-Met, MaeII) and 1001A-G (294Lys-Arg, MboII) according to GenBank accession number NM _214282, were detected by using PCR-RFLP. Some of them belonged to the rich-cysteine immuno protein domains as thrombospondin type I repeats (TSP1), membrane-attack complex/perforin (MACPF) and complement control protein (CCP). Genotypic trequencyat SNPs 990C-T and 1001A-G was in a strong agreement with Hardy-Weinberg equilibrium for populations. In addition, the pC7 was assigned to chromosome 16q 14 (Retention traction 38 percent, linked marker soon, 21 cR in distance, LOD = 17.94) by using IMpRH mapping. Statistically significant difference was found for 1001A-G genetic association with CH50 (p=0.008) and C3c (p=0.0405). The results promote the porcine C7 gene as a candidate gene for improvement of disease resistance and porcine health, one of the main targets of animal breeding program.
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