Parkinson's disease (PD) is a progressive neurodegenerative movement disorder. Nowaday, several studies have found mutant forms of genes linked to PD and UCH-L1 is a protein that plays an important role in ubiquitin-proteasome system, it also found in Lewy bodies of PD patients so that UCH-L1 may be related to the development of late-onset idiopathic PD but its function was poorly understood. In the other hand, 70 percent of known human disease genes match in the genome of fruit flies Drosophila melanogaster so many sciencetists use it as a model to do research on human diseases, the regulation of gene expression and the link between various genes; among them, numerous Drosophila cell culture lines (i.e., S2, Kc, BG2-C6, etc.) have been established and biochemical studies can be performed. The most recent additions to the arsenal of tools available to Drosophila researchers are RNA interference (RNAi). RNAi is a phenomenon whereby dsRNA induces the degradation of homologous messenger RNAs (mRNAs), thereby depleting the protein encoded by the targeted mRNA. In orther to investigate the role ofUCH-Li in PD, the authors constructed plasmid pBluescrip KS II (+) carrying Cduch-11 (420bp). After checking, the authors utilized it to make dsRNA and examined the ability of knocking down dUCH-L1 in Schneider (S2) cells of Drosophila melanogaster. The level of duch-11 mRNA and protein dUCH-L1 were examined by Realtime PCR and Western Blot with anti dUCH-L1 antibody, the authors found the decreasement of dUCH-L1 level from 0h to 8h.
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