Rice is one of the most important crop plants over the world. the work studied on the physiological responses of rice seedlings (Oryza sativa cv. Dongjin) under drought condition by withholding water. In response to drought treatment, three-week-old rice seedlings exhibited typically morphological responses as leafs rolling and drying at 48 h after exposure to water deficit. Together with the loss of water relation (relative water content and shoot water potential), reactive oxygen species (H2O2 and malondialdehyde level) accumulated in the leaves of rice seedlings which led to apparent reduction in photosynthetic efficiency but only slight reduction of chlorophyll content.