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Lượt truy cập
 Lượt truy cập :  14,894,442

76.03

Công nghệ sinh học trong y học

Nguyễn Thị Hoa; Vũ Thị Hiền; ứng Thị Diệu Thúy; Nguyễn Quang Liêm; Đinh Duy Kháng; Đồng Văn Quyền; Nguyễn Quang Liêm(1)

Nghiên cứu tạo phức hợp Chromatophore - chấm lượng tử (Quantum Dots) để tạo Biosensor phát hiện các tác nhân gây bệnh

Generation of complex of CdTe quantum dot and chromatophore - The essential requirement for biosensor development

Y học Việt Nam

2012

2

124-128

1859-1868

Công nghệ nano; Chromatophore; Chấm lượng tử; Biosensor

Following the research on producion of antibody against recombinant Beta-subunit of FOF1-ATPase, in this study, the authors reported the generation of complex of chromatophore extracted from Rhodospirillum rubrum with quantum dots; these are essential requirements for biosensor development. R. rubrum cells were disrupted by French pressure combines ultrasonication to release the chromatophore. Chromatophore was purified by density-gradient ultracentrifugation. The pigmented bands were collected and examined by electron microscope with magnification of 15,000X - 30,000X. The results showed that chromatophore shaped like discs and cups 100 nm in diameter. The highly purified chromatophore was then mixed with red QDs, 2-3 nm in size, in suitable conditions to form complex of chromatophore-QDs. The formation of complexes was confirmed by luminescence intensity under fluorescent microscope. The complex will be used for further research to develop the biosensor.

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