GA20 oxidases are important enzymes in the biosynthetic pathway of bioactive GA - phytohormones. Each gene encoding GA20 oxidase was expressed specifically in an organ or at defferent stages of plant development. GA20 oxidase gene was widely used in plant genetic transformation. Previously, we have showed the result of isolation and sequencing of GA20 promoter and GA20 oxidase gene from A. mangium. In Vietnam, the GA20 oxidase gene was also isolated and sequenced from Arahidopsis for the purpose of genetic engineering of plants. In this paper, the authors reported the construction of two new recombinant Ti-plasmid pCB301-Kan harbouring the GA20 promoter and GA20 oxidase gene., The authors carried out transient expression experiments of the GA20 oxidase gene in leaves of N. tahacum. The first vector, pCB301-35Sp-SP-GA20, contains sequences of 35S promoter, GA20 oxidase gene and signal peptide leading expressed protein into endoplasmic reticulum. The second vector, pCB301-GA20p-GA20, carries full-length sequence of GA20 promoter and the GA20 oxidase gene. The GA20 oxidase gene in two recombinant vectors has been expressed transiently in leaves of N. tahacum both at the levels of transcription and translation. By fusing a coding sequence of c-myc peptide with sequence of GA20 oxidase gene, it is convenient to check the expression of GA20 oxidase gene via Western blot. The fusion protein produced in transient expression experiments confirmed that the expression vectors functioned in the model plant cells. So these vectors can be used for stable transformation.
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