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Lượt truy cập
 Lượt truy cập :  15,409,073

76.29.54

Y học lâm sàng

Nguyễn Thị Mai Phương; Trần Vân Khánh; Nguyễn Trọng Tuệ; Trần Thị Oanh; Hoàng Đạo Bảo Trâm; Nguyễn Thị Hà; Tạ Thành Văn; Nguyễn Trọng Tuệ(1)

Định lượng vi khuẩn Porphyromonas gingivslis trong viêm nha chu bằng kỹ thuật Real Time PCR

Quantification of porphyromonas gingivalis bacteria by real-time PCR technique

Nghiên cứu y học

2014

3

17-26

0868-202X

Viêm nha chu; Porphyromonas gingivslis; Định lượng; Kỹ thuật PCR; Nghiên cứu

This study was to quantify Porphyromanas gingiva/is in chronic and aggressive periodontitis patients in comparison with healthy subjects by qPCR, qPCR has been used to quantify Parphyroman as gingivalis in gingival cervical fluid in both disea~ed sites of periodontal patients and healthy sites of healthy subjects. The comparison were done in both diseased sites of chronic periodontitis patients P. gingiva lis 25.10 +/- 4.68 Cycle threshold (Ct) and healthy subjects (P. gingiva/is 33.93 +/- 3.17 Ct) respectively (p 0.05). The comparison were in both diseased sites of aggressive periodontitis patients (P. gingivalis 22.89 +/- 4.63 Ct) and healthy subjects (P. gingivalis 34.38 +/- 2.96 Ct) respectively (p 0.01). In conclusion, Porphyromonas gingiva lis was very common in both chronic and aggressive periodontitis patients (100 percent) and there was a significant difference of Porphyromonas gingivalis quantity in comparison with healthy subjects (p 0.05).

TTKHCNQG, CVv 251